Publications

Merlicek, L. P.; Neumann, J.; Lear, A.; Degiorgi, V.; de Waal, M.; Cotet, T.-S.; Mulholland, A. J.; Bunzel, H. A.: AI.zymes – A modular platform for evolutionary enzyme design. bioRxiv: the preprint server for biology, 2025.01.18.633707 (2025)

Schnettler, J. D.; Wang, M. S.; Gantz, M.; Bunzel, H. A.; Karas, C.; Hollfelder, F.; Hecht, M. H.: Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins. Nature Chemistry 16 (7), pp. 1200 - 1208 (2024)

Jabeen, H.; Beer, M.; Spencer, J.; van der Kamp, M. W.; Bunzel, H. A.; Mulholland, A. J.: Electric fields are a key determinant of carbapenemase activity in class A β-lactamases. ACS Catalysis 14 (9), pp. 7166 - 7172 (2024)

Fröhlich, C.; Bunzel, H. A.; Buda, K.; Mulholland, A. J.; van der Kamp; W., M.; Johnsen, P. J.; Leiros, H.-K. S.; Tokuriki, N.: Epistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase. Nature Catalysis 7 (5), pp. 499 - 509 (2024)

Buzas, D.; Bunzel, H. A.; Staufer, O.; Milodowski, E. J.; Edmunds, G. L.; Bufton, J. C.; Mateo, V.; V, B.; Yadav, S. K. N.; Gupta, K. et al.; Fletcher, C.; Williamson, M. K.; Harrison, A.; Borucu, U.; Capin, J.; Francis, O.; Balchin, G.; Hall, S.; Vega, M. V.; Durbesson, F.; Lingappa, S.; Vincentelli, R.; Roe, J.; Wooldridge, L.; Burt, R.; Anderson, R. J. L.; Mulholland, A. J.; Group, B. U.; Hare, J.; Bailey, M.; Davidson, A. D.; Finn, A.; Morgan, D.; Mann, J.; Spatz, J.; Garzoni, F.; Schaffitzel, C.; Berger, I.: In vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2. Antibody Therapeutics 6 (4), pp. 277 - 297 (2023)

Lear, A.; Anderson, J. L. R.; Hilvert, D.; Arcus, V. L.; van der Kamp, M. W.; Bunzel, H. A.; Mulholland, A. J.: Comment on: “Computer simulations reveal an entirely entropic activation barrier for the chemical step in a designer enzyme”. ACS Catalysis 13 (15), pp. 10527 - 10530 (2023)

Hutchins, G. H.; Noble, C. E. M.; Bunzel, H. A.; Williams, C.; Dubiel, P.; Yadav, S. K. N.; Molinaro, P. M.; Barringer, R.; Blackburn, H.; Hardy, B. J. et al.; Parnell, A. E.; Landau, C.; Race, P. R.; Oliver, T. A. A.; Koder, R. L.; Crump, M. P.; Schaffitzel, C.; Oliveira, A. S. F.; Mulholland, A. J.; Anderson, J. L. R.: An expandable, modular de novo protein platform for precision redox engineering. Proceedings of the National Academy of Sciences 120 (31), e2306046120 (2023)

Bunzel, H. A.; Smith, J. A.; Oliver, T. A. A.; Jones, M. R.; Mulholland, A. J.; Anderson, J. L. R.: Photovoltaic enzymes by design and evolution. bioRxiv: the preprint server for biology, 2022.12.20.521207 (2022)

Hindson, S. A.; Bunzel, H. A.; Frank, B.; Svistunenko, D. A.; Williams, C.; van der Kamp, M. W.; Mulholland, A. J.; Pudney, C. R.; Anderson, J. L. R.: Rigidifying a de novo enzyme increases activity and induces a negative activation heat capacity. ACS Catalysis 11 (18), pp. 11532 - 11541 (2021)

Bunzel, H. A.; Anderson, J. L. R.; Hilvert, D.; Arcus, V. L.; van der Kamp; W., M.; Mulholland, A. J.: Evolution of dynamical networks enhances catalysis in a designer enzyme. Nature Chemistry 13 (10), pp. 1017 - 1022 (2021)

Stenner, R.; Bunzel, H. A.; Mulholland, A. J.; Anderson, R. L. J.: Beneficial substrate partitioning boosts non-aqueous catalysis in de novo enzyme-alginate beads. bioRxiv: the preprint server for biology, 2021.04.12.439416 (2021)

Bunzel, H. A.; Anderson, J.L. R.; Mulholland, A. J.: Designing better enzymes: Insights from directed evolution. Current Opinion in Structural Biology 67, pp. 212 - 218 (2021)

Basler, S.; Studer, S.; Zou, Y.; Mori, T.; Ota, Y.; Camus, A.; Bunzel, H. A.; Helgeson, R. C.; Houk, K. N.; Jiménez-Osés, G. et al.; Hilvert, D.: Efficient Lewis acid catalysis of an abiological reaction in a de novo protein scaffold. Nature Chemistry 13 (3), pp. 231 - 235 (2021)

Otten, R.; Pádua, R. A. P.; Bunzel, H. A.; Nguyen, V.; Pitsawong, W.; Patterson, M.; Sui, S.; Perry, S. L.; Cohen, A. E.; Hilvert, D. et al.; Kern, D.: How directed evolution reshapes the energy landscape in an enzyme to boost catalysis. Science 370 (6523), pp. 1442 - 1446 (2020)

Kries, H.; Bloch, J. S.; Bunzel, H. A.; Pinkas, D. M.; Hilvert, D.: Contribution of oxyanion stabilization to Kemp eliminase efficiency. ACS Catalysis 10 (8), pp. 4460 - 4464 (2020)

Bunzel, H. A.; Kries, H.; Marchetti, L.; Zeymer, C.; Mittl, P. R. E.; Mulholland, A. J.; Hilvert, D.: Emergence of a negative activation heat capacity during evolution of a designed enzyme. Journal of the American Chemical Society 141 (30), pp. 11745 - 11748 (2019)

Bunzel, H. A.; Garrabou, X.; Pott, M.; Hilvert, D.: Speeding up enzyme discovery and engineering with ultrahigh-throughput methods. Current Opinion in Structural Biology 48, pp. 149 - 156 (2018)