Equipment Flow Cytometry

BD Fortessa Flow Cytometer
Laser lines: 445 nm, 488 nm, 514 nm, 561 nm. 13 fluorescence channels and high-throughput sampler (for measurements in multi-well plates)

BD FACSAria Fusion
Laser lines: 355 nm, 445/488 nm, 561 nm, 633 nm. 18 fluorescence channels. 2 or 4 way sorting. Single cell sorting into multi-well plates.

Applications

• Measurements of single-cell gene expression in populations, including analysis of gene expression noise
• Measurements of dose-dependent activation of transcriptional reporters in multi-well plates
• Cell cycle studies in prokaryotic and eukaryotic microorganisms
• Cell Sorting